Test for Lipids Using Sudan Iii Solution

Sudan III
A sample of dye Sudan III
Sudan III
Names
Preferred IUPAC name

1-{[4-(Phenyldiazenyl)phenyl]diazenyl}naphthalen-2-ol

Other names

Sudan Red BK, Fat Ponceau G, Cerasin Red, C.I. 26100, Solvent Red 23, Solvent Red 164, Sudan Red, Sudan Red III, Sudan V, Sudan Red B, Sudan G, Scarlet B, and Tony Red

Identifiers

CAS Number

  • 85-86-9 check Y

3D model (JSmol)

  • Interactive image
ChEBI
  • CHEBI:82535 check Y
ChEMBL
  • ChEMBL2001927
ChemSpider
  • 16736189 check Y
ECHA InfoCard 100.001.490 Edit this at Wikidata
KEGG
  • C19527 check Y

PubChem CID

  • 62331
UNII
  • ND733RX3JN check Y

CompTox Dashboard (EPA)

  • DTXSID3041742 Edit this at Wikidata

InChI

  • InChI=1S/C22H16N4O/c27-21-15-10-16-6-4-5-9-20(16)22(21)26-25-19-13-11-18(12-14-19)24-23-17-7-2-1-3-8-17/h1-15,27H/b24-23-,26-25+check Y

    Key: FHNINJWBTRXEBC-HXEHWPEMSA-Ncheck Y

  • InChI=1/C22H16N4O/c27-21-15-10-16-6-4-5-9-20(16)22(21)26-25-19-13-11-18(12-14-19)24-23-17-7-2-1-3-8-17/h1-15,27H/b24-23-,26-25+

    Key: FHNINJWBTRXEBC-HXEHWPEMBU

SMILES

  • Oc4ccc1ccccc1c4/N=N/c3ccc(\N=N/c2ccccc2)cc3

Properties

Chemical formula

C 22 H 16 N 4 O
Molar mass 352.397 g·mol−1
Melting point 199 °C (390 °F; 472 K)
Hazards[1]
Safety data sheet Sigma-Aldrich Sudan III
GHS pictograms GHS05: Corrosive GHS07: Exclamation mark
GHS Signal word Warning

GHS hazard statements

H315, H318, H319, H335, H413

GHS precautionary statements

P101, P102, P103, P261, P264, P270, P271, P273, P280, P302+P352, P304+P340, P305+P351+P338, P310, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, P501

Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

☒ Nverify (what is check Y ☒ N  ?)
Infobox references

Chemical compound

Sudan III is a lysochrome (fat-soluble dye) diazo dye. It is structurally related to azobenzene.[2]

Uses [edit]

It is used to color nonpolar substances such as oils, fats, waxes, greases, various hydrocarbon products, and acrylic emulsions. Its main use is as a fuel dye in the United States of America mandated by the Internal Revenue Service (IRS) to distinguish low-taxed heating oil from automotive diesel fuel, and by the United States Environmental Protection Agency (EPA) to mark fuels with higher sulfur content; it is a replacement for Solvent Red 26 with better solubility in hydrocarbons.[3] The IRS requires "a concentration spectrally equivalent to at least 3.9 pounds of... Solvent Red 26 per thousand barrels of fuel" (11.1 mg/l);[4] the concentrations required by EPA are roughly 5 times lower.

Biological staining [edit]

Sudan III is a dye used for Sudan staining. Similar dyes include Oil Red O, Sudan IV, and Sudan Black B. They are used for staining of triglycerides in frozen sections, and some protein bound lipids and lipoproteins on paraffin sections. It has the appearance of reddish brown crystals and a maximum absorption at 507(304) nm.[5]

Safety [edit]

Sudan I, Sudan III, and Sudan IV have been classified as category 3 carcinogens by the International Agency for Research on Cancer.[6]

References [edit]

  1. ^ "Safety Data Sheet Sudan III". fishersci.com . Retrieved 24 August 2020.
  2. ^ Hunger, Klaus; Mischke, Peter; Rieper, Wolfgang; Raue, Roderich; Kunde, Klaus; Engel, Aloys (2005). "Azo Dyes". Ullmann's Encyclopedia of Industrial Chemistry. Weinheim: Wiley-VCH. doi:10.1002/14356007.a03_245.
  3. ^ "Archived copy". Archived from the original on 2005-05-10. Retrieved 2006-02-02 . CS1 maint: archived copy as title (link)
  4. ^ "Publication 510: Excise Taxes (Including Fuel Tax Credits and Refunds)" (PDF). IRS.gov. February 2020. p. 9. Retrieved February 10, 2021.
  5. ^ R. D. Lillie. Conn's Biological Stains. Williams & Wilkins, Baltimore, MD., U.S.A.
  6. ^ Refat NA, Ibrahim ZS, Moustafa GG, Sakamoto KQ, Ishizuka M, Fujita S (2008). "The induction of cytochrome P450 1A1 by Sudan dyes". J. Biochem. Mol. Toxicol. 22 (2): 77–84. doi:10.1002/jbt.20220. PMID 18418879. S2CID 206010951.
  • Susan Budavari, Editor, (1996). The Merck Index, Ed. 12. Merck & Co., Inc., Whitehouse Station, NJ, USA
  • Edward Gurr, (1971). Synthetic Dyes in Biology, Medicine and Chemistry. Academic Press, London, England.

External links [edit]

  • Stains File entry

Test for Lipids Using Sudan Iii Solution

Source: https://en.wikipedia.org/wiki/Sudan_III

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